| 1 | African University of Humanities and Applied Sciences | Surt | Tripolitania |
| 2 | Al-Jafra University | Al Jufrah | Fezzan |
| 3 | Al-Rafaq National University | Surt | Tripolitania |
| 4 | Al-Tahadi National Medical University | Surt | Tripolitania |
| 5 | Alasmarya Islamic University | Marqab | Tripolitania |
| 6 | Azzaytuna University | Marqab | Tripolitania |
| 7 | Bani Waleed University | Misrata | Tripolitania |
| 8 | Bright Star University | Al Wahat | Cyrenaica |
| 9 | Elmergib University | Marqab | Tripolitania |
| 10 | Fezzan University | Murzuq | Fezzan |
| 11 | Islamic University of Mohamed Bin Ali Al Sanussi | Al Butnan | Cyrenaica |
| 12 | Libyan Academy for Postgraduate Studies | Misrata | Tripolitania |
| 13 | Libyan International University | Banghazi | Cyrenaica |
| 14 | Libyan University of Humanities and Applied Sciences | Surt | Tripolitania |
| 15 | Misurata University | Misrata | Tripolitania |
| 16 | Nalut University | Nalut | Tripolitania |
| 17 | Omar Al-Mukhtar University | Al Jabal al Akhdar | Cyrenaica |
| 18 | Sabratha University | Az Zawiyah | Tripolitania |
| 19 | Sebha University | Sabha | Fezzan |
| 20 | Sirte University | Surt | Tripolitania |
| 21 | Tobruk University | | Cyrenaica |
| 22 | Tripoli National University | Tarabulus | Tripolitania |
| 23 | University of Ajdabia | Al Wahat | Cyrenaica |
| 24 | University of Alhadera | Al Butnan | Cyrenaica |
| 25 | University of Benghazi | Banghazi | Cyrenaica |
| 26 | University of Gharyan | Al Jabal al Gharbi | Tripolitania |
| 27 | University of Tripoli | Tarabulus | Tripolitania |
| 28 | University of Zawia | Az Zawiyah | Tripolitania |
| 29 | Zintan University | Al Jabal al Gharbi | Tripolitania |
